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Title: GENE PROFILING OF MARC-145 CELLS INFECTED WITH PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS USING CROSS-SPECIES MICROARRAY HYBRIDIZATION

Author
item Fox, James
item Chitko-Mckown, Carol

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/21/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: PRRSv infections within the U.S. can account for up to 15-20% economic losses yearly in the swine industry. Such losses result from PRRSv pathogenicity as well as opportunistic bacterial infections that result from impairment of the pulmonary defense mechanisms as a result of PRRSv infection. Limited information is available about the host cell response to oinfection by these viral agents. Examining this response is necessary to gain a better understanding of the dynamics of the virus-cell interactions. Identifying responsive host genes to PRRSv infection and determining whether they are pathogen- or cell-induced will provide clues into understanding the dynamics of the infection and may provide insight into intervention strategies. To this end, we have used cross-species microarray hybridization (UNIGem 2.0, Incyte Genomics) to profile gene expression responses in PRRSv infected MARC-145 cells. Because we desired to examine the dynamics of this response, we analyzed infected cells at 2, 6, 10 16, and 24 hours post-infection. Infected cells were compared to mock infected cells that were similarly treated. As expected, many genes expression was altered upon PRRSv infection. Interestingly, subsets of genes were identified that responded early after infection, while other subsets were identified that responded late after infection. Both up- and down-regulated genes were identified and their absolute differential expression displayed up to 13-fold differences between infected vs. uninfected cells.