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Title: CONSTRUCTION OF A MOLECULAR LINKAGE MAP OF WILD OAT (AVENA FATUA L.) FOR IDENTIFICATION OF QTLS GOVERNING DORMANCY

Author
item NADELLA, K - NORTH DAKOTA STATE UNIV.
item Hoffer, Barry
item Foley, Michael

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/25/2001
Publication Date: 1/12/2002
Citation: Nadella, K.D., Hoffer, B.L., Foley, M.E. 2002. Construction of a molecular linkage map of wild oat (Avena fatua L.) for identification of QTLs governing dormancy. [Abstract]. Plant, Animal, & Microbe Genomes X Conference. p. 182.

Interpretive Summary: Wild oat (Avena fatua) is a serious weed in small grain crops. Variable germination due to seed dormancy contributes to its persistence and spread. To improve the control of wild oat, we are investigating genetic and environmental factors regulating dormancy. To this end, 126 F8:F9 RI lines derived from the cross 'M73' (D) x 'SH430' (ND) are being used to develop a genetic linkage map of wild oat and to detect dormancy QTLs. To date, 150 AFLP loci have been mapped in a subset of 81 RI lines and 27 linkage groups have been identified covering 580 cM. Germinability of RI line seeds is being determined at temperatures from 5 to 30 C and significant effect of temperature on germinability have been observed. Using bulked segregant analysis, we have discovered 10 loci linked to dormancy at 15 C. Of these, three 'M73'-derived loci were clustered on linkage group 20, and one locus each from 'SH430' and 'M73' were mapped to linkage groups 12 and 16, respectively. The association of these markers with dormancy is statistically significant. Additional AFLP and RFLP loci will be used to construct a saturated genetic map on the entire population and to align with the cultivated hexaploid oat map. QTLs segregating for dormancy in the population will be mapped and their interaction with germination temperature will be investigated.

Technical Abstract: Wild oat (Avena fatua) is a serious weed in small grain crops. Variable germination due to seed dormancy contributes to its persistence and spread. To improve the control of wild oat, we are investigating genetic and environmental factors regulating dormancy. To this end, 126 F8:F9 RI lines derived from the cross 'M73' (D) x 'SH430' (ND) are being used to develop a genetic linkage map of wild oat and to detect dormancy QTLs. To date, 150 AFLP loci have been mapped in a subset of 81 RI lines and 27 linkage groups have been identified covering 580 cM. Germinability of RI line seeds is being determined at temperatures from 5 to 30 C and significant effect of temperature on germinability have been observed. Using bulked segregant analysis, we have discovered 10 loci linked to dormancy at 15 C. Of these, three 'M73'-derived loci were clustered on linkage group 20, and one locus each from 'SH430' and 'M73' were mapped to linkage groups 12 and 16, respectively. The association of these markers with dormancy is statistically significant. Additional AFLP and RFLP loci will be used to construct a saturated genetic map on the entire population and to align with the cultivated hexaploid oat map. QTLs segregating for dormancy in the population will be mapped and their interaction with germination temperature will be investigated.