Submitted to: American Society for Virology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 3/14/2001
Publication Date: N/A
Technical Abstract: The variability of North American and Asian isolates of Soybean mosaic virus (SMV) was investigated in two separate experiments. In the first, coat protein (CP) encoding regions of the genomes of 38 SMV isolates from North America, China, and Korea were evaluated by restriction fragment length polymorphism (RFLP) analysis of cDNA fragments amplified by reverse transcriptase polymerase chain reaction. Even though differences were detected among isolates, their geographic origin could not be distinguished. In a second set of experiments, variability in nucleotide and predicted amino acid sequences corresponding to portions of the Pl, helper component/protease (HC/Pro), and CP regions of 18 of the isolates was assessed. Of the three regions, the CP predicted amino acid and nucleotide sequences were the most similar among the isolates and produced phylogenetic relationships similar to those obtained from the RFLP analyses. The HC/Pro and PI sequences were more variable, with the PI region being the most variable of the three. Analysis of these two regions separated the 18 isolates into two distinct groups based on their geographic origins. Chinese SMV isolates showed much larger amounts of genetic diversity than North American isolates in the Pl and HC/Pro regions. The relatively low diversity of North American SMV isolates could be due to a founder effect of the introduction of a small number of SMV isolates into North America from Asia.