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United States Department of Agriculture

Agricultural Research Service

Title: GENERATION OF A SUBGROUP J AVIAN LEUKOSIS VIRUS INFECTIOUS CLONE EXPRESSING GREEN FLUORESCENCE PROTEIN AND ITS POTENTIAL USES)

Author
item Lupiani, Blanca
item Hunt, Henry
item Conklin, Kathleen
item Reed, Willie
item Fadly, Aly

Submitted to: North Central Avian Disease Conference
Publication Type: Abstract only
Publication Acceptance Date: 10/2/2001
Publication Date: N/A
Citation:

Interpretive Summary:

Technical Abstract: During the last decade, subgroup J avian leukosis virus (ALV-J) has been associated with serious mortality and other production problems in meat-type chickens world wide. ALV-J isolates have been shown to be very variable both at the genetic and antigenic level. In this report, we describe the generation of an infectious molecular clone of strain ADOL-R5-4 of ALV-J (pR5-4). This molecular clone is in the form of proviral DNA and is infectious upon transfection into chicken cells. pR5-4 has also been modified to include a splice acceptor site after the viral env gene as well as a unique restriction site that allows the cloning of foreign genes into the virus (pR5-4 SA). In order to test the stability of this viral clone we have cloned a soluble modified form of the green fluorescence protein (smGFP) into this unique site in pR5-4 SA. The resultant plasmid, pR5-4 SA smGFP, has been shown to be very stable even after 3 month of passaging in tissue culture. This new molecular clone is an essential tool in our attempts to study cell tropism of ALV-J in both meat- and egg-type chickens.

Last Modified: 8/24/2016
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