Author
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Laegreid, William |
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Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 8/2/2000 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Accurate detection of cattle infected with E. coli O157:H7 is important for both research and regulatory purposes. The development of improved culture and other methodologies has been hindered by the lack of a "gold standard" for E. coli O157:H7 detection by which to evaluate detection techniques. Our lab has developed a culture methodology that detects up to 85% more E. coli O157:H7 infected cattle than other commonly used methods. Furthermore, the effects of sample size and sample handling on apparent prevalence of E. coli O157:H7 infected cattle were examined and found to significantly affect apparent prevalence. Using the best available detection methodology is imperative to ascertain the magnitude of the E. coli O157:H7 problem in cattle, to determine the possible sources of infection and to evaluate intervention strategies |
