Skip to main content
ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #125685
Title: ANALYSIS OF NAHMS TONSIL SAMPLES FOR THE PRESENCE OF AIL-BEARING YERSINIA ENTEROCOLITICA USING A 5' NUCLEASE FLUOROGENIC PCR (TAQMAN) ASSAY

Author
item BYL, L - IOWA STATE UNIVERSITY
item Wesley, Irene

Submitted to: Food Safety Consortium Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 9/18/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The fluorogenic 5' nuclease PCR (TaqMan) assay, which was developed earlier in this laboratory, was further optimized and used to screen hogs for Yersinia enterocolitica. Tonsil samples (n = 1,647) collected during the National Animal Health Monitoring System (NAHMS) Swine 2001 program were tested for presence of the ail gene, which is present in virulent Y. enterocolitica. Tonsil swabs were collected on farms (n = 101) located in Arkansas, Colorado, Illinois, Indiana, Iowa, Kansas, Michigan, Minnesota, Missouri, Nebraska, North Carolina, Ohio, Oklahoma, Pennsylvania, South Dakota, Texas, and Wisconsin. Of the 1,647 tonsil samples, 11.4% (n = 188) were positive.