|Van Campen, H|
Submitted to: International Positive Strand RNA Virus Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 5/28/2001
Publication Date: N/A
Citation: Interpretive Summary:
Technical Abstract: Four genotypes are recognized within the pestivirus genus. They are bovine viral diarrhea virus (BVDV) genotype 1, BVDV genotype 2, classical swine fever virus (CSFV) and border disease virus (BDV). In addition, unique pestiviruses have been isolated from wildlife. A pestivirus isolated from giraffe most probably represents an additional genotype, however, classification of pestiviruses isolated from bison and reindeer in Europe in incomplete. In this study a virus, isolated from a blind antelope that had histological lesions similar to mucosal disease syndrome, was characterized. Antigenic characterization using polyclonal and monoclonal antibodies raised against BVDV1 and BVDV2 suggested this virus was antigenically related but distinct from BVDV genotypes. Similarities at the genomic level indicated that this virus belongs to the pestivirus genus. These similarities include the organization of the 5' UTR, the presence of a N**PRO coding region, conservation of predicated amino acid sequences flanking the cleavage sites between viral polypeptides N**PRO and C and between C and E**RNS and conservation of predicted hydrophobicity plots of structural proteins. While this data indicates the virus belongs to the pestivirus genus, phylogenetic analysis suggests that it is the most divergent of the pestivirus identified to date. Comparison of predicted N amino acid sequences from viruses from recognized pestivirus genotypes show similarity values that range between 64.9% to 76.2%. Similar comparisons between the antelope virus sequence and viruses from the recognized pestivirus genotypes yielded similarity values that ranged between 55.4% and 61.3%. These results suggest that this virus represents first member of a new pestivirus genotype.