Submitted to: ASA-CSSA-SSSA Annual Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/21/2001
Publication Date: 10/21/2001
Citation: KYNAST, R.G., OKAGAKI, R.J., ODLAND, W.E., PHILLIPS, R.L., RINES, H.W. INHERITANCE OF MAIZE CHROMOSOMES IN OAT-MAIZE PARTIAL HYBRIDS. ASA-CSSA-SSSA ANNUAL MEETING ABSTRACTS. 2001. ABSTRACT. P. 361.
Technical Abstract: F1 plants recovered from oat x maize crosses have usually retained all 21 oat chromosomes but only occasionally possess maize chromosomes. The number and identity of maize chromosomes eliminated/retained in F1 hybrids may depend on oat x maize genotype interaction. We recovered all ten maize chromosomes as single additions to haploid oat (n = 3x+1 = 22) among F1 hybrids generated from more than 60,000 oat x maize crosses and have made available to the scientific community genomic DNA of the additions. Haploid oat with one or more maize chromosome(s) can produce doubled haploid additions among F2 offspring owing to frequent meiotic restitution. This natural restoration of partial fertility or that provided by colchicine doubling and/or somatic endopolyploidization resulted in 38 fertile F2 offspring with maize chromosomes 1, 2, 3, 4, 5, 6, 7, 8, and 9 each as disomic additions to hexaploid oat (2n = 6x+2 = 44). Here we report on specific phenotypic characters as well as maize chromosome transmission of these additions. We demonstrate the usefulness of each addition line for maize genetics and genomics, in particular for mapping genes and markers to chromosome. This material is based upon work supported by the National Science Foundation under Grant No. 9872650.