Submitted to: International Society of Citriculture Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 7/15/2000
Publication Date: 12/15/2000
Citation: KRUEGER, R., GULSEN, O., ROOSE, M.L. USE OF MOLECULAR MARKERS IN MANAGEMENT OF CITRUS GERMPLASM RESOURCES. INTERNATIONAL SOCIETY OF CITRICULTURE ABSTRACTS. 2000.
Technical Abstract: Molecular markers have been used in the management of plant genetic resources for some time. Their use in the management of genetic resources of perennial crops, particularly citrus, is somewhat different than for seed crops. The use of molecular markers has been initiated in the management of citrus genetic resources maintained in Riverside, CA by the US Dept of Agriculture and the University of California . Molecular markers have been used to identify nucellar seedlings (representing the type) for accessions received as seed, to reduce the number of tree maintained by identifying redundancies, identify accessions for which the identification had been lost, and to screen for pathogens. Examples of these uses are given. Ongoing projects include surveying the field collection and assessing the amount of genetic diversity actually present. This will permit designation of a core collection, prioritization of accessions for evaluation and other activities, and indicate redundancies and gaps in the collection. The markers utilized have been SSR and ISSR, which offer several advantages over other types of markers in these applications. Other potential uses and benefits of molecular markers in the maintenance and management of citrus genetic resources are discussed.