Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/2/2001
Publication Date: N/A
Technical Abstract: Hydroxy fatty acids are considered important industrial materials because the hydroxyl group gives fatty acids special properties such as higher viscosity and reactivity compared with non-hydroxy fatty acids. These hydroxy fatty acids are classified into mono-, di-, and tri-hydroxy fatty acids. Among those fatty acids, 9,10,13-trihydroxy-11E-octadecenoic acid (9,10,13-THOD),9,12,13-trihydroxy-10(E)-octadecenoic acid (9,12,13-THOD), and 9,10,11-trihydroxy-12(E)-octadecenoic acid (9,10,11-THOD) have gained special attention because they were isolated from rice plants with strong anti-fungal activity. Although the biological significance of these THODs was reported, their production in nature is restricted to mostly plant systems, and in trace amounts. Intense efforts were focused to develop microbial system for the production of these hydroxy fatty acids. Previously, we reported that a novel bacterial strain Pseudomonas aeruginosa PR3 converted linoleic acid to two compounds: 9,10,13-THOD and 9,12,13-THOD (Kim H, HW Gradner and CT Hou. 2000. J Ind Microbial Biotechnol 25:109-115). In this study we report the effect of several metal ions as catalytic agents of lipid peroxidation on the production of total THODs by PR3. Among ten different metal ions tested, Fe**+2 and CU**+2 were effective for producing THODs but bacterial growth was not significantly affected by types of metal ions tested. Minimal concentration of FE**+2 and CU**+2 ions for comparable production of THODs were 0.1 mM and 0.05 mM, respectively.