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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #124343
Title: COMPARATIVE EVALUATION OF SALMONELLA DETECTION ASSAYS IN SWINE FECES

Author
item ROSTAGNO, M - FEDERAL UNIV OF LAVRAS
item Hurd, Howard
item Gailey, Jared
item MCKEAN, J - IOWA STATE UNIVERSITY
item LEITE, R - FEDERAL UNIV MINAS GERAIS

Submitted to: International Symposium on Epidemiology and Control of Salmonella in Pork
Publication Type: Proceedings
Publication Acceptance Date: 9/5/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The performance of four commercially available Salmonella detection assays were comparatively evaluated for detection of Salmonella in swine fecal samples. The detection assays included two antigen capture enzyme-linked immunoassays (ELISA 1 and 2), a DNA hybridization assay, and an immunochromatographic assay. For the "gold-standard," the combination of results from two isolation methods were used. Briefly, both methods included pre-enrichment (Tetrathionate or Rappaport-Vassiliadis broth), enrichment in Rappaport-Vassiliadis broth, and plating on XLT-4 agar. The sensitivity and agreement (Kappa statistics) with the "gold-standard" for each evaluated detection assay were: 87.36% and 0.64 for the ELISA 1; 98.85% and 0.96 for the ELISA 2; 97.70% and 0.92 for the DNA hybridization assay; and 80.46% and 0.48 for the immunochromatographic assay. From these results, it can be concluded that there are good Salmonella detection assays currently available, that could be useful in investigations using clinical samples, like swine feces.