|Guill, Katherine - Kate|
Submitted to: Plant Molecular Biology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/1/2001
Publication Date: 3/1/2002
Citation: SHAROPOVA, N., MCMULLEN, M.D., SCHULTZ, L., SCHROEDER, S., SANCHEZ-VILLEDA, H., GARDINER, J., BERGSTROM, D., HOUCHINS, K.E., MELIA-HANCOCK, S., MUSKET, T. DEVELOPMENT AND MAPPING OF SSR MARKERS FOR MAIZE. PLANT MOLECULAR BIOLOGY. 2002. V. 48(5). P. 463-481. Interpretive Summary: Efficient molecular markers aid crop genetics research and crop improvement. The classes of marker known as simple sequence repeat (SSR) markers are particularly cost effective for researchers of both small and high-throughput scale. However, the limited number of publicly available SSR markers has been a limiting factor in many areas of corn research. The eobjectives of this study were to develop and map SSR markers for corn and release this information to the scientific community through MaizeDB (www.agron.missouri.edu/ssr.html). We have developed and genetically mapped over 1000 new SSR markers. These markers benefit both public and private corn researchers, particularly in the genetic analysis of complex agronomic traits for corn.
Technical Abstract: Microsatellite or simple sequence repeat (SSR) markers have wide applicability for genetic analysis in crop plant improvement strategies. The objectives of this project were to isolate, characterize, and map a comprehensive set of SSR markers for maize (Zea mays L.). We developed 1051 novel SSR markers for maize from microsatellite-enriched libraries and dby identification of microsatellite-containing sequences in public and private databases. Three mapping populations were used to derive map positions for 978 of these markers. The main mapping population was the intermated B73 x Mo17 (IBM) population. In mapping this intermated recombinant inbred line population, we have contributed to development of a new high-resolution map resource for maize. The primer sequences, original sequence sources, data on polymorphisms across 11 inbred lines, and map positions have been integrated with information on other public SSR markers sand released through MaizeDB at URL:www.agron.missouri.edu. The maize research community now has the most detailed and comprehensive SSR marker set of any plant species.