Author
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Allen, Patricia |
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Submitted to: Coccidiosis International Conference Proceedings
Publication Type: Abstract Only Publication Acceptance Date: 5/10/2001 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Changes in analytes that might indicate infection-induced oxidative stress in the chick host were surveyed during Eimeria acervulina and Eimeria tenella infections. Chicks were infected at 3 weeks of age. At 8, 30, 48, 72, 96, 120, 150, 170, and 240 hours post inoculation (HPI), blood as well as spleen, liver and infected gut tissue samples were taken for analysis. Similar samples were taken from uninfected controls at 30, 72, 120, 170 and 240 HPI. Significant increases in plasma NO2-+ NO3- during both infections were noted from 30 HPI, and peak levels were coincident with periods of maximum weight gain depression. Whole blood GSH, GSSG as well as GSH/GSH+GSSG declined throughout both infections indicating states of oxidative stress lasting beyond the period of maximum weight gain depression and maximum NO production. In infected duodenal tissue, levels of both GSH and GSSG were significantly reduced between 72 and 240 HPI, possibly a consequence of absorptive mucosal destruction and reduced recycling of bile GSH. Few changes in cecal GSH were observed. Decreased GSH and GSH/GSH+GSSG in spleen at 8 and 30 HPI were the earliest sign of redox imbalance during both infections. But, GSH synthesis was evidently upregulated in spleen and liver subsequently. It is concluded that both E. acervulina and E. tenella infections initiate states of oxidative stress in the chick that are manifested by increased production of the free radical, NO . , and changes in GSH/GSSG balances in spleen, liver and gut. |