Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract only
Publication Acceptance Date: 3/14/2001
Publication Date: 3/14/2001
Citation: DAVIS, G., MUSKET, T., MELIA-HANCOCK, S., DURU, N., SHAROPOVA, N., SCHULTZ, L., MCMULLEN, M.D., SANCHEZ, H., SCHROEDER, S., GARCIA, A. THE INTERMATED B73 X MO17 GENETIC MAP: A COMMUNITY RESOURCE. MAIZE GENETICS CONFERENCE. 2001. ABSTRACT. P. 69. Interpretive Summary:
Technical Abstract: A high-resolution corn genetic map was constructed in a B73 x Mo17 population derived after four generations of random-mating. Theoretically, the random-mating process increases the number of recombination events present in an individual mapping line by 3-fold. Our data support this. One thousand low copy number restriction fragment length polymorphism m(RFLP) loci and more than 700 simple sequence repeat (SSR) loci were mapped. Screening images of the RFLPs and SSRs are available at http://www.agron.missouri.edu/images.html. A table detailing the primer information for the SSRs is available at http://www.agron.missouri.edu/ssr.html. The SSR primers are derived from public EST sequence data and from enriched libraries produced in-house. The RFLPs include many markers mapped on other public populations. In addition, novel Pstl clones and Mu-tagged clones have been added to the map. The map has better local order resolution than prior public maps because of the increased number of recombinants per individual. It serves as a bridge between prior public maps and the maize physical map currently under construction. A subset of lines from the mapping population has been selected for researchers who want to map a gene of interest. The lines are available from the Maize Genetics Cooperation Stock Center and will be available as DNA in microtiter plates for PCR-based assays from the Missouri Maize Project.