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Title: INSERTIONAL MUTAGENESIS OF A PUTATIVE IMMUNOGENIC EPITOPE OF PP38 RISPENS STRAIN OF MAREK'S DISEASE VIRUS INTO MD5

Author
item Lee, Lucy
item CUI, ZHIZHONG - YANGZHOU UNIVERSITY PRC
item Coulson, Barry
item Reddy, Sanjay

Submitted to: American Veterinary Medical Association Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/14/2001
Publication Date: 7/14/2001
Citation: LEE, L.F., CUI, Z., COULSON, B.D., REDDY, S.M. INSERTIONAL MUTAGENESIS OF A PUTATIVE IMMUNOGENIC EPITOPE OF PP38 RISPENS STRAIN OF MAREK'S DISEASE VIRUS INTO MD5. AMERICAN VETERINARY MEDICAL ASSOCIATION ABSTRACT. 2001.

Interpretive Summary:

Technical Abstract: Marek's disease virus (MDV) phosphorylated protein pp38 is one of the unique MDV proteins abundantly expressed in serotype 1 MDV and tumor cells. Substitution of one amino acid in Rispens pp38 resulted in reduced immune responses in chickens upon inoculation with the mutant virus. Using three monoclonal antibodies for pp38, we have identified a third epitope related to immunogenicity of pp38 of Rispens strain. Using overlapping cosmid technology we have switched the pp38 gene of Md5 with that of Rispens. Monoclonal antibody specific for Rispens pp38 was used to detect the expression of pp38 gene of Rispens in Md5.