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Title: DEVELOPMENT OF A SUBGENOMIC PROMOTER-DRIVEN CUCUMBER MOSAIC VIRUS-BASED VECTOR

Author
item Zhao, Yan
item Hammond, John
item Hammond, Rosemarie

Submitted to: American Phytopathological Society
Publication Type: Abstract Only
Publication Acceptance Date: 5/15/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Cucumber mosaic virus (CMV), the type member of the genus Cucumovirus, can infect over 800 plant species representing 365 genera and 85 families. Because of its broad host range, CMV shows significant potential as a vector for delivery and expression of foreign genes in plants. RNA3 of the CMV Ixora strain (subgroup I), which carries the movement protein (MP) and coat protein (CP) genes, was modified to include an additional subgenomic promoter for expression of a reporter gene, the green fluorescent protein (GFP) from jellyfish. The engineered subgenomic promoter, followed by the reporter gene, was positioned between the MP gene and the inter-cistronic region (IR) upstream of the CP gene. Two types of constructs were made to test the efficiency and stability of the tricistronic vector: one involves the simple duplication of the native CP subgenomic promoter and the other employs the insertion of a subgenomic promoter from a subgroup II strain and is aimed at reducing the frequency of homologous recombination. The constructs are currently being evaluated in tobacco protoplasts as well as in whole plants.