Submitted to: European Journal of Lipid Science and Technology
Publication Type: Review Article
Publication Acceptance Date: 3/13/2001
Publication Date: N/A
Technical Abstract: While Ag-HPLC is a powerful technology for separating fatty acid isomers, the large number of TAG isomers present in conjugated linoleic acid (CLA)-containing triacylglycerol (TAG) formulations has limited its use in their analysis. Another problem is the low concentrations of CLA in the milk, meat and plasma (0.1% to only 2.0% by weight) of humans and animals. Silver-ion thin layer chromatography has been used to fractionate complex TAG mixtures. The TAG fractions are then usually converted to fatty acid esters (methyl, methoxy, phenacyl, etc.) before further analysis by reverse-phase or Ag-HPLC, GC, FT-IR, etc. Ag-HPLC has found limited use in analysis of seed oil TAGs containing higher concentrations (40-60%) of conjugated fatty acids. In the future, Ag-HPLC should find increasing usage in the analysis of seed oil samples and for characterization of less-complex CLA-containing TAG formulations for patent applications or CLA-containing TAG diet supplements.