|Van Campen, H|
Submitted to: American Society for Virology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 7/20/2001
Publication Date: N/A
Citation: N/A Interpretive Summary:
Technical Abstract: Four genotypes are recognized within the pestivirus genus [bovine viral diarrhea virus (BVDV) genotype 1, BVDV genotype 2, classical swine fever virus (CSFV), and border disease virus (BDV)]. In this study, a unique virus with pestivirus characteristics was isolated from a blind pronghorn. Binding of polyclonal and monoclonal antibodies raised against BVDV1 and BVDV2 suggested this virus was antigenically related to, but distinct from, both BVDV genotypes and BDV. Genomic characteristics unique to pestiviruses indicated that this virus belonged to the pestivirus genus. These characteristics included the length and organization of the 5' UTR, the presence of a Npro coding region, conservation of predicted amino acid sequences flanking the cleavage sites between viral polypeptides Npro and C and between C and Erns, and conservation of predicted hydrophobicity of structural proteins. While this data indicated the virus belonged to the pestivirus genus, phylogenic analysis suggested that it was very different from any of the pestiviruses characterized to date. Comparison of predicted Npro amino acid sequences of viruses from recognized pestivirus genotypes show similarity values that range from 64.9% to 76.2%. Similar comparisons of Npro coding sequences yielded similarity values that ranged from 55.4% to 61.3%. These results suggest this virus represents the first member of a new pestivirus genotype. It also represents the first pestivirus genotype associated with New World wildlife.