Submitted to: International Plant and Animal Genome IX Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/26/2001
Publication Date: 1/26/2001
Citation: Preiszer, J., Vantoai, T.T., Huynh, T., Bolla, R., Yen, H. 2001. Structure and activity of a soybean adh promoter determined in transgenic hairy roots. International Plant and Animal Genome IX Conference. Interpretive Summary:
Technical Abstract: The alcohol dehydrogenase (Adh) gene in plants is transciptionally induced by anoxia and hypoxia. The Adh gene family consists of one to four members depending on the plant species. The developmental expression and tissue-specific responses of each gene member to hypoxic stress have been well documented. In addition to hypoxia, the Adh gene is also induced by heat, dehydration, cold and treatment with the hormone ABA. Extensive studies of the structure and function of the maize Adh promoters have identified several common motifs that are essential for the proper responses to environmental stimuli. Compared to maize and Arabidopsis, the structure and expression of the soybean Adh promoter have not been as well documented. In this study, we isolated a 976 bp fragment upstream of the soybean Adh2 Gene start codon. The putative promoter contains motifs homologous to the Anaerobiosis Responsive Element (ARE) and the G-box-1 palindromic element characteristic of known Adh promoters. The putative promoter was fused with the GUS reporter gene and the pBI-AdhGUS plasmid was introduced into soybean cotyledons by Agrobacterium rhizogenes transformation. GUS activity assay and histochemical staining of the five transgenic AdhGUS hairy roots indicated that the promoter was inducible by anoxia but did not respond to cold temperature, wounding and ABA treatment. GUS expression in transgenic 35S-GUS hairy roots was not affected by anoxia, cold, wounding and ABA. The intention is to use the promoter in transformation experiments where the hypoxically induced root specific expression of the transgenes is needed to improve the plants tolerance to flooding or hypoxia stress.