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Title: EVALUATION OF ENTOMOPATHOGENIC NEMATODES AGAINST THE BLOWFLY-LUCILIA SERICATA, A CASUAL AGENT OF MYIASIS

Author
item TOTH, E - EOTVOS UNIVERSITY
item MARIGLIGETI, K - EOTVOS UNIVERSITY
item FODOR, A - EOTVOS UNIVERSITY
item LUCSKAI, A - VESZPREM UNIVERSITY
item FARKAS, R - SZT ISTVAN UNIVERSITY
item Klein, Michael

Submitted to: International Insects Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 1/11/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Myiasis is the infestation of vertebrate animals by dipterous larvae. The sheep blowfly Lucilia sericata, a facultative ectoparasite in the family Calliphoridae, is the primary agent of sheep myiasis in temperate north-western Europe, parts of southern Africa, Australia and New Zealand. Defense against myiasis causing flies is difficult and the use of biological pest control is likely to expand. The following entomopathogenic nematode species were tested against the 2nd stage L. sericata: 12 strains of Heterorhabditis bacteriophora (IS5, HHU1, HMo1, HNC1, HAZ36, HBrecon, HHU2, HAZ29, HHP88, HHU3, HHu4, HGua); Steinernema intermedia, S. glaseri NC513, S. anomali, S. riobrave, Steinernema sp. and 5 S. feltiae strains (SF22, DSF Vija Norway, SFHU1, SFscp, SFIS6). Analysis of fly mortality was evaluated using the Mentel-Haenszel test method of NCSS 97 program in which the odds ratios were calculated for each treatment/sample/repeat together with control, for the effect of temperature and all odds ratio results were investigated for significance by Fisher's exact test. None of the nematodes gave positive result at 37 deg C. At other temperatures, only S. feltiae strains produced mortality of L. sericata larvae. Strain SFHU1 had the most marked effect, equally at 20 deg C and 25 deg C. The second best was SF22, but the elevated temperatures reduced mortality. Strain SF Vija Norway caused significant mortality at 20 20 deg C but no infection at 25 deg C. Results of IS6 were opposite to Vija Norway, as the 5 deg C temperature rise increased the odds ratio values for contracting disease to a significant level. With SFscp, the odds ratios could not be calculated.