Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 12/15/2000
Publication Date: 1/13/2001
Citation: KARAKOUSIS, A., GUSTAFSON, J.P., CHALMERS, K. THE INTEGRATION OF SSR MARKERS INTO CONSENSUS MAPS OF AUSTRALIAN BARLEY MAPPING POPULATIONS. PLANT AND ANIMAL GENOME. 2001. ABSTRACT. P. 126. Interpretive Summary:
Technical Abstract: Australian barley researchers and more recently the National Barley Molecular Marker Program (NBMMP) have made considerable progress in mapping loci controlling traits of agronomic importance. Much of this progress has been achieved through the genetic analysis of the five major double- haploid mapping populations: Galleon x Harruna Nijo (popn=112), Chebec x Harrington (popn=120), Clipper x Sahara (popn=150), Alexis x Sloop (popn=111), and Amaji Nijo x WI2585 (popn=139). These maps are extensively covered with RFLP markers and some with AFLP markers. In the past, RFLP markers identified from these maps have been used on a routine basis in marker assisted selection (MAS) programs and aided in the development of new barley varieties. SSR (microsatellite) markers have now been integrated into these maps and barley consensus maps have been generated using the computer software package Joinmap V 2.0. This map integrates 143 SSR markers with existing RFLP and AFLP markers. It has been recently used to aid in the identification of 62 SSR markers linked within 10 cm to 14 previously identified Australian agronomic traits. The identification of these markers linked to these important agronomic traits will substantially reduce the expense and technical complexity in MAS programs, while increasing the mapping efficiency relative to RFLP markers. This consensus map will play a major role in assisting barley breeders to select SSR markers of importance to Australian breeding programs.