Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/13/2001
Publication Date: 1/13/2001
Citation: BUSHMAN, B.S., MCMULLEN, M.D., GERKE, J., SNOOK, M.E., BERHOW, M.A., ROEBKE, C.J., HOUCHINS, K.E., MAURER, A., SZALMA, S.S. GENETIC ANALYSIS OF CHLOROGENIC ACID SYNTHESIS IN MAIZE. PLANT AND ANIMAL GENOME. 2001. ABSTRACT P. 173.
Technical Abstract: Chlorogenic acid is reported to have a wide range of biological effects, from insect resistance to human health. Chlorogenic acid is a product of the phenylpropanoid biosynthetic pathway and shares substrates with lignins, anthocyanins, flavones, isofavonoids, and other secondary metabolites. To determine significant genetic components for chlorogenic acid biosynthesis, such as allelic variation of pathway enzymes or regulators, we have employed quantitative trait analyses (QTL) on three F2 populations derived from three maize inbred liens: A619, Mp708, and Mo6. The silk tissue of A619 accumulates negligible chlorogenic acid, while Mp708 and Mo6 synthesize much larger amounts than are usually present in inbred lines. Resulting QTLs have been ascribed to candidate genes of the phenylpropanoid pathway where possible. p1, a transcription activator of certain flavonoid enzymes, was found to exert large effects on chlorogenic acid biosynthesis. A QTL on chromosome 2 common to all three populations also exerted a large effect, but associates with no candidate gene. QTLs that may represent phenylalanine ammonia lyase (pal) alleles were detected on chromosomes 4 and 5. These analyses provide clues to significant pathway regulators and control points on which to focus functional studies.