Skip to main content
ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #116748
Title: EVALUATION OF CROSS-SPECIES HYBRIDIZATION WITH PORCINE MRNA TO HUMAN MICROARRAYS

Author
item FITZSIMMONS, C - IOWA STATE UNIVERSITY
item MARKLUND, L - IOWA STATE UNIVERSITY
item Stabel, Thomas
item GALINA, L - PIC GROUP, FRANKLIN, KY
item TUGGLE, C - IOWA STATE UNIVERSITY

Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 1/17/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Measuring gene expression using microarray technology has the potential to become an important tool to find genes that control economic traits in livestock. Opportunities to use this new technology are limited because of the lack of porcine-specific microarrays. One alternative is to attempt cross-species hybridizations to microarrays of human and/or rodent origin. In this experiment, pigs were inoculated intranasally with Salmonella choleraesuis (n=4) or saline (n=4), and necropsied 7 days post- challenge. Total RNA was prepared from pooled lung and spleen samples within each treatment group. These RNAs were combined to create "infected" versus "control" spleen/lung RNA mixtures. This combination of RNA was sent to Incyte Genomics on two separate occasions to be hybridized to their UniGEM V**TM human microarray (7075 unique clones), and was determined by Incyte to be of sufficient quality. The total number of genes that passed quality standards was 80% and 38% in the first and second hybridizations, respectively. The number of genes that were up-regulated at least 1.8-fold in the first hybridization was 23 (0.3%), as compared to 16 genes (0.2%) in the second. The number of genes down-regulated at least 1.8-fold in the first hybridization was 61 (0.9%), as compared to 2 (0.03%) in the second. There was no agreement between the genes identified as differentially expressed between experiments. Real-time PCR tests were also developed to compare expression results of the microarray. Variability between hybridizations and cost of the array were too great to warrant further use of this microarray by our laboratory.