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Title: FATTY ACIDS ENHANCED TUBERMYCIN PRODUCTION BY PSEUDOMONAS STRAIN 2HS

Author
item KEUDELL, K
item HUANG, J
item WEN, L
item KLOPFENSTEIN, W
item BAGBY, MARVIN
item Lanser, Alan
item Plattner, Ronald
item PETERSON, R
item Weisleder, David

Submitted to: Microbios
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/10/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary: A new microorganism from sheep manure produced a known antibiotic, tubermycin B. This antibiotic prevents the growth of microorganism which causes tuberculosis in human beings. An interesting finding was that when these microorganisms were growing in nutrient broth and the fatty acid derived from agriculture commodity was presented, the production of this antibiotic by this microorganism was increased more than 70 fold (from 1 liter nutrient broth, we can get 192 mg this antibiotic). Fatty acid enhanced microorganism production of this antibiotic has never been reported. This information will be useful to those studying microbial transformation for enhancing yields of this antibiotic.

Technical Abstract: A new microbial isolate, Pseudomonas 2HS, produced trace amounts of a greenish-yellow pigment when grown aerobically in a 1% yeast extract medium at 30C and shaken at 250 rpm for 5 days. In contrast, cells produced more greenish- yellow pigment (2.16 mg/15 ml culture) when grown in the presence of 0.5% 12-hydroxy-octadecanoic acid (w/v). The greenish-yellow pigment was identified as phenazine-1- carboxylic acid (tubermycin B), and the Pseudomonas 2HS was identified as P. aeruginosa 2HS. This is the first report that 12-hydroxyoctadecanoic, ricinoleic and other fatty acids can enhance the production of phenazine-1-carboxylic acid by a Pseudomonas species.