Submitted to: Journal of Animal Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/21/2001
Publication Date: N/A
Interpretive Summary: This paper describes the genetic mapping of seven cattle genes onto the bovine linkage map. These markers can be used to provide information to compare the human and cattle maps, supporting efforts to identify genes that control production traits in livestock. The particular seven genes were chosen because they contain a type of marker called a microsatellite within the expressed part of the gene, and these microsatellite markers are particularly convenient for use with conventional equipment.
Technical Abstract: Primers were developed from expressed sequence tags (ESTs) obtained from bovine mammary gland, small intestine, brain and pituitary cDNA libraries. The EST database was screened for the presence of microsatellites using a search algorithm to identify dinucleotide or trinucleotide repeat units, and flanking primers were designed to amplify across these repeats in 150-200 bp amplicons. Genotypes were collected by incorporation of radionucleotides during PCR and sizing of alleles on polyacrylamide gels. Two-point linkage analysis was performed with the genotypes of the MARC population, using CRI-MAP. The number of informative meioses, and maximum LOD score for two- point analysis with markers in the indicated linkage groups are given in Table 2. Multipoint analysis positioned each marker within its linkage group. The bovine chromosome and position, measured in cM from the most centromeric marker in the linkage group, are shown in Table 2. Five of the EST sequences reported here were obtained from the 5' end of the transcript; however, none of the sequences showed significant (score > 300) similarity to known genes in the GenBank database via BLAST analysis. These microsatellites therefore represent markers for genes expressed in various bovine tissues, although the nature and function of the loci are not currently known.