Submitted to: Journal of Agricultural Genomics
Publication Type: Peer reviewed journal
Publication Acceptance Date: 7/1/1999
Publication Date: 1/20/2000
Citation: Hoffman, D.L., Hang, A., Burton, C.S. 2000. Interval mapping of aflp markers for barley using a subset of doubled-haploid lines. Journal of Agricultural Genomics v.5 article 4. Interpretive Summary: Barley is an important cereal grain for food, beverage, and feed. As with other major crops, detailed genetic maps have been developed for barley using the techniques of molecular biology. Such maps are used to locate and tag genes controlling traits related to quality as well as other economic traits such as disease resistance. The marker tags are used to efficiently monitor the transfer of such genes into high yielding, adapted varieties. The original molecular maps were primarily based on solid, yet laborious and slow procedures. We demonstrated that markers derived from a more modern and efficient procedure can readily be assigned to an original barley map with a select subset of the original mapping population. This information will be used by scientists looking for fast and efficient markers to help enhance the quality of barley, whether it be for food, beverage, or feed.
Technical Abstract: Molecular markers have been used to map the barley genome and locate loci controlling quantitative traits. The amplified fragment length polymorphism (AFLP) procedure has been used to map some barley populations, but not that of 'Steptoe'/'Morex', a reference mapping population of North America. Fourteen EcoRI/MseI AFLP primer pairs generated 1,120 DNA fragments between 60 and 330 base pairs in length. Of these, 226 fragments were polymorphic between Steptoe and Morex. Using a subset of 15 segregating doubled-haploid lines, 148 AFLP markers were readily assigned to chromosome intervals on the Steptoe/Morex RFLP map. On most of the chromosomes, clustering of markers near the centromeres was observed, and a few sizable gaps were noted. These patterns were similar to those observed in the interval placement of PCR-RAPD markers. As with PCR-RAPD markers, some AFLP markers were more reliable than others, and this quick mapping procedure provides a means for identifying the more reliable markers for future studies.