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ARS Home » Research » Publications at this Location » Publication #113130
Title: INCORPORATION OF SEQUENCED CDNA AND GENOMIC MARKERS INTO THE SOYBEAN MAP

Author
item Matthews, Benjamin - Ben
item Devine, Thomas
item WEISEMANN, JANE
item LIN, JHY-JHU - LIFE TECHNOLOGIES, INC.
item KUO, JONATHAN - LIFE TECHNOLOGIES

Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/1/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary: Our knowledge of the soybean genome is growing rapidly and promises to be an invaluable tool for improving soybean quality and yield. We are building a map of the soybean genome using DNA sequences representing known genes and using phenotypic traits such as root fluorescence, seed coat color, and flower color, resistance to soybean mosaic virus and resistance to bacterial pustule infection. In this report, we focus on the genetic mapping of genes of known function and several of questionable or unknown function. Our soybean map includes genomic markers used in other laboratories, so it can be compared to and aligned with other soybean maps containing these markers. This map will be useful to soybean breeders using marker assisted selection to identify plants containing desirable traits and to molecular biologists who wish to study genome structure, organization and gene function.

Technical Abstract: The soybean [Glycine max (L.) Merr.] expressed sequence tagged (EST) database is growing rapidly and promises to be a valuable resource for discovering agronomically important genes. Genetic maps featuring cDNAs of known sequence and function are important, because association of genes with phenotypes will increase understanding of the molecular mechanisms affecting valuable agronomic traits. Our objective is to place sequenced cDNA (EST) and genomic clones on an anchored soybean genetic map. The genetic mapping of these markers was conducted using standard restriction fragment length polymorphism (RFLP) techniques with an F2 population of 149 individuals derived from a cross between two publicly available soybean genotypes cv. Noir 1 (PI 290136) and cv. BARC-2 (Rj4) (PI 547895). DNA sequences of mapped EST and genomic clones were compared with accessions in GenBank, and significant sequence similarities are reported. Because the objective was to map ESTs and sequenced genomic clones, only the 24 linkage groups (1200 cM) containing the 39 mapped EST and sequenced genomic clone markers plus the four phenotypic traits root fluorescence (Fr2), seed coat color (I), flower color (W1) and nodulation response (Rj4) were presented. Amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) markers were added to increase marker density. Simple sequence repeat (SSR) markers were included to align this map with other soybean maps.