Submitted to: Biochemical Systematics and Ecology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/1/2001
Publication Date: 3/5/2002
Citation: LI, X., RALPHS, M.H., GARDNER, D.R., WANG, R. GENETIC VARIATION WITHIN AND AMONG 22 ACESSIONS OF THREE TALL LARKSPUR SPECIES (DELPHINIUM SPP.) BASED ON RAPD MARKERS. BIOCHEMICAL SYSTEMATICS AND ECOLOGY. 2002.
Technical Abstract: Random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in three species of toxic larkspurs (Delphinium spp). A total of 184 plants from five western states were analyzed by 23 RAPD primers that amplified 188 reproducible bands. There were 144 polymorphic bands; 10 shared by Delphinium glaucum and D. occidentale, 8 shared by D. barbeyi and dD. glaucum, and 18 shared by D. occidentale and D. barbeyi. Thirteen band were specific for D. occidentale, 18 for D. glaucum and 19 for D. barbeyi. There were 58 bands that were specific for individual accessions and 44 bands that were common to all three species. Using the144 polymorhpic RAPD bands, a dendrogram was generated to assess the genetic similarity among the samples. The cophenetic values were 0.64 between D. occidentale and D. barbeyi, and 0.55 between the cluster of these two species and D. glaucum. These relationships are congruent with those based on morphological characters and support the contention that these are separate species.