Submitted to: Animal Genetics International Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/14/2000
Publication Date: N/A
Technical Abstract: Alpha mannosidase 2B2 is being investigated as a potential candidate gene for ovulation rate in swine located on chromosome 8. Exon 1 was sequenced from the 20 grandparents of the MARC resource population. Four polymorphisms were detected; three of which were in the 5' UTR. The fourth polymorphism was a C/T transition in the putative start codon as reported in GenBank Accession number D28521. Another ATG, which could serve as a start codon, is located in the same open reading frame 33 bp downstream. The predominant allele contained a C at this position indicating that the latter start codon is the most frequently used. Three of the grandparents (two Meishan and one White Composite) were C/T heterozygotes. The remaining animals were homozygous for the C allele. An assay to detect this polymorphism was design by microsequencing the region and determining the alleles via MALDI-TOF mass spectrometry. Selected animals from the third through fifth generations of the MARC resource population were genotyped. The mean ovulation rate was 14.19 +/- 2.52 for the C/T heterozygote (n = 36) and 14.48 +/- 2.58 for the C/C homozygote (n = 312). Only one animal was determined to be homozygous for the T allele due to the low frequency in our population and no phenotype was available. The polymorphism in the putative start codon of alpha mannosidase 2B2 does not appear to be influencing ovulation rate.