Submitted to: Genome Research
Publication Type: Peer reviewed journal
Publication Acceptance Date: 9/20/2000
Publication Date: N/A
Citation: Interpretive Summary: In the last 15 years there has been increased efforts by scientists to apply biotechnology to better understand the genetic nature of organisms. One of these areas of understanding that has benefited greatly by the application of biotechnology is construction of genetic maps. Genetic maps are created by the analysis of DNA in organisms. The analysis of DNA yields information about the position of genes (made of DNA) on chromosomes (the inherited elements during procreation). Genes are located on chromosomes in linear arrays (one gene followed by another). If the location of several genes is known then their positional (on the chromosome) relationships can be determined. If the relationships between genes is known (genetic map), then scientists can better understand and use this information for the more efficient improvement of plants. In cucumber several genetic maps have been produced using various biotechnological methods. It would be important to join these maps to produce one unified consensus map which provides a complete picture of the position of all the genes that have been discovered. Therefore, we developed and applied new biotechnological methods to produce a consensus map in cucumber. This map will allow plant breeders to know the position of economically important genes which will improve the efficiency of producing commercial hybrid and inbred lines with improved yield and quality. The consumer will directly benefit from such improvements as costs of production will decrease.
Technical Abstract: Thirty four polymorphic SSRs were evaluated for length polymorphism in melon (Cucumis melo L.) and cucumber (C. sativus L.). SSR markers were located on 3 melon maps: (a) 18 on the map of 'Vedrantais' and PI 161375; (b) 23 on the map of 'Piel de Sapo' and PI 161375 and ? 16 on the map of PI 414723 and 'Dulce'. In addition, 14 of the markers were located on the cucumber map of GY14 and PI 183967. SSRs were proved to be randomly distributed throughout the melon and cucumber genomes. Mapping of the SSRs in the different maps led to the cross identification of seven linkage groups in all melon maps. In addition, 9 SSRs were common to both melon and cucumber maps. The potential of SSR markers as anchor points for melon map merging and for comparative mapping with cucumber was demonstrated.