Author
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Deahl, Kenneth |
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GOTH, ROBERT - FORMERLY OF VEGETABLE LAB |
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Submitted to: Potato Association of America Proceedings
Publication Type: Abstract Only Publication Acceptance Date: 3/16/1999 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: The pathogenicity of late blight oospores was determined on detached leaves of potato. We excised primary leaves from the 3rd to the 6th node of sterile healthy plants and inserted their petioles into floral aqua tubes containing 9ml of sterile distilled water. Leaves in the aqua tubes were placed on galvanized metal hardware cloth (12.5 x 12.5 mm mesh) and positioned 2.5 cm above distilled water (2.5 cm depth) in 31 cm x 17 cm x 8 cm clear plastic boxes with tight fitting lids. The plastic containers were kept in growth chambers at 16C and 16 hour photo period of mixed fluorescent and incandescent light. Single germinating oospores were placed in the center of the terminal leaflet in a droplet of water on each test leaf. Eight days after an inoculation, characteristic late blight symptoms developed on the inoculated leaflets. This detached-leaf method was used to demonstrate the abilities of single-oospore offspring to infect, colonize and sporulate on potato leaves. Although the importance of sexual recombination in establishing and maintaining diversity is unclear and presumptive, this technique could be utilized in efforts to delineate the precise role of oospores in the disease and life cycles of this organism. |