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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #110028
Title: IDENTIFICATION OF SSR MARKERS LINKED TO ROOT NECROSIS GENES IN SOYBEAN (GLYCINE MAX)

Author
item HEIL, A - IOWA STATE UNIVERSITY
item MIN, X - IOWA STATE UNIVERSITY
item Palmer, Reid

Submitted to: Iowa Academy of Science
Publication Type: Abstract Only
Publication Acceptance Date: 2/1/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: There have been four recessive mutations found in soybean that cause disease lesions mimicking Phytophthora root rot infection. These mutations are allelic and designate a new locus Rn for Root Necrosis. The Rn phenotype displays a browning of the roots at 5-7 days, which correlates to oxidation of phenolic compounds and an increased amount of phytoalexins. The increased amount of phytoalexins is consistent with a hypersensitive response to wounding or pathogen attack. These responses may lead to programmed cell death that is morphologically related to apoptosis in animal cells. To identify the Rn locus, two bulks of soybean leaves were constructed from the F2 generation in which one was the homozygous dominant and the other the recessive. SSR then was used to screen the bulks and determine markers that are linked to the Rn gene. These markers were used to screen the entire F2 population so a map distance between the RN gene and the marker could be determined.