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item Brodie, Bill

Submitted to: American Journal of Potato Research
Publication Type: Abstract Only
Publication Acceptance Date: 7/23/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: During routine evaluation of potato clones and varieties from meristem cultures to confirm their resistance to Globodera rostochiensis pathotype Ro1, we discovered that cysts developed on Atlantic NewLeaf clone 6 of the Ro1 resistant cultivar Atlantic that had been transformed with the Bt gene. We obtained a clone of the original nontransformed Atlantic that was used in the transformation and the transformed Atlantic clones 6, 31, and 36 that were from different transformation events. RFLP analysis of these clones indicated that all of them possessed the marker for the H1 gene that confers resistance to G. rostochiensis Ro1. Bioassays in which the plants were subjected to the nematode in pot tests revealed that G. rostochiensis developed freely on Atlantic NewLeaf clone 6 but not on clones 31 and 36 or on the original nontransformed Atlantic clone. These data indicate that the Bt transformation process affected the expression of the H1 gene in clone 6 6of Atlantic NewLeaf but not in the other transgenic clones. This finding highlights the importance of evaluating transformed plants for any previously known resistance traits before they are selected for commercialization.