Submitted to: Academic Press
Publication Type: Review Article
Publication Acceptance Date: 6/1/2000
Publication Date: N/A
Technical Abstract: It is important to convert vegetable oils and animal fats into value- added industrial products. One of such products is oxygenated fatty acid. Oxygenated metabolites of fatty acids also play a variety of important roles in biological systems. We have been investigating the production of monohydroxy and multihydroxy fatty acids from vegetable oils and their component fatty acids through biocatalysis. Microbial enzymes can produce not only monohydroxy but also di- or tri-hydroxy fatty acids. We discovered that microbial enzymes produced 7,10- dihydroxy-8(E)-octadecenoic acid (DOD) from oleic acid and 12,13,17- trihydroxy-9(Z)-octadecenoic acid (THOA) from linoleic acid. We also found that Pseudomonas aeruginosa PR3 system converted ricinoleic acid to a new compound, 7,10,12-trihydroxy-8(E)-octadecenoic acid. The structures of these trihydroxy unsaturated fatty acids resemble those of plant self-defense substances. Microbial production of trihydroxy unsaturated fatty acids are new. On physiological activity of these hydroxy fatty acids, we found that DOD inhibited the growth of Candida albican but did not inhibit the growth of A. flavus, A. fumigatus, C. neoformans, and M. infracellulare. We also found that at 200 ppm concentration, THOA inhibited the growth of some plant pathogenic fungi such as wheat powdery mildew and wheat leaf rust.