Author
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CAO, XIAOLING - CONTRACT EMPLOYEE |
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Hammerschlag, Freddi |
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Submitted to: HortScience
Publication Type: Abstract Only Publication Acceptance Date: 6/20/2000 Publication Date: 6/20/2000 Citation: Cao, X., Hammerschlag, F.A. Growth regulator pretreatments significantly enhance the efficiency of shoot organogenesis from leaf explants of highbush blueberry cultivar bluecrop. Hortscience. 35:445 Interpretive Summary: Technical Abstract: As part of a program to develop transgenic highbush blueberry (Vaccinium corymbosum L.) cultivars, studies were conducted to determine optimum conditions for high efficiency shoot regeneration from leaf explants of in vitro-propagated, commercially important, tissue culture-recalcitrant Bluecrop' shoot cultures. The effects of pretreatments, growth regulators and age of explant source on shoot organogenesis were investigated. A maximum of 98 % shoot regeneration and 10 shoots regenerating per leaf explant occurred when explants of 2-week-old shoot cultures were incubated in the dark (for a total of 14 days) on pretreatment medium #1 containing 2.6 uM NAA and 5 uM TDZ for 4 days, next on pretreatment medium #2 containing 2.6 uM NAA and 7 uM zeatin riboside for 3 days, then on regeneration medium containing 1 uM TDZ for 6 weeks, and last on medium without growth regulators for 10 days. No shoot regeneration occurred if explants were incubated without exposure to pretreatments prior to incubation regeneration medium. There were no significant differences in percentage of regeneration or the number of shoots regenerating per explant from leaf explants derived from either 1-, 2-, or 3-week-old shoot cultures. Shoot production per explant on 1 uM TDZ was about three times that on either 0.5 uM TDZ or 20 uM zeatin riboside, and nine times that on 5 uM TDZ. |