Submitted to: American Society for Microbiology
Publication Type: Abstract Only
Publication Acceptance Date: 5/25/2000
Publication Date: N/A
Technical Abstract: Previous phylogenetic studies revealed that Bacillus sphaericus sensu lato is a composite of seven or more unrelated clades, one of which is B. sphaericus and another B. fusiformis. The taxonomic position of two of the remaining groups was examined. DNA relatedness analyses showed that the two groups are distinct from each other, from B. sphaericus, B. fusiformis, and from the other unidentified clades. The genetic isolation of the two groups was further shown by differences in their whole cell fatty acid compositions and by their separate clustering in dendrograms based on numerical analyses of phenotypic data obtained using the BIOLOG system. The two groups share with all other B. sphaericus-like organisms the inability to grow anaerobically, inability to use common hexoses as sources of carbon, and G+C values of 34 to 36 mol percent. Unique characteristics of group 1 include the ability to utilize Beta- hydroxybutyrate and propionate, the nonrequirement for vitamins, and the inability to grow in 5 percent NaCl. In contrast, group 2 utilizes alanylamide, AMP, and UMP, requires biotin and thiamin for growth, and grows in the presence of 5 percent NaCl. The requirement for cystine is a characteristic that distinguishes group 2 from all the other groups. These data show that groups 1 and 2 represent two new species for which the names Bacillus pycnoticus and Bacillus neidii are proposed.