Submitted to: Journal of Animal Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/10/2000
Publication Date: N/A
Interpretive Summary: A useful genetic marker was developed and placed on the swine genomic map. This marker was localized to position 105 cM on the USDA-MARC linkage map and bands 14-15 on the long arm of chromosome 6. This new marker may help researchers identify economically important genes in the future.
Technical Abstract: A single 10-bp oligonucleotide having the sequence 5'-GTA GAC GAG C-3' was used to PCR amplify a 255 bp fragment from porcine genomic DNA. The PCR amplification of the single 10 bp oligonucleotide produced a 'plus/present' or 'minus/absent' polymorphism. The plus/present band (255 bp) was sequenced for identification. This marker displayed a dominant marker phenotype. Segregation of the polymorphic product was observed in eight two-generation USDA-MARC Swine Reference Families based on assumption of a two-allele dominant system represented by 'presence' or 'absence' of PCR product. Individuals with no PCR product were assumed to be homozygous for the minus allele. Genotypes for the 104 animals of the USDA-MARC Swine Reference Families were determined by agarose gel resolution of PCR products and included in linkage analysis with the porcine USDA-MARC linkage map. The two-point linkage analysis using CRIMAP 2.4 has identified a chromosome 6 (SSC6) region with linked microsatellite markers (S0228 with LOD=5.45, SW2098 with LOD=5.01 and SWR726 and SW824 with LOD=4.99). PCR analysis of 27 porcine-rodent somatic cell hybrids using the DK122 SCAR primers and conditions listed above gave positive results for hybrids 9,12,18,19, and 26, which indicated assignment of DK122 to porcine chromosome 6(1/2 p14)-p15.