|Goodwin, Stephen - Steve|
Submitted to: Plant and Animal Genome Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 1/8/2000
Publication Date: N/A
Technical Abstract: As part of a project to analyze the functional genomics of pest resistance in wheat, an EST database was constructed from the cultivar P29. Random cDNA clones from a non-normalized library made from nine-day old seedling tissue were sequenced at the Purdue University Genome Facility. Blast analyses of the first 254 ESTs from the database revealed abundant messages for genes involved in photosynthesis, as well as more unusual sequences. Approximately 25% of the ESTs corresponded to rubisco, 5% to rubisco activase, 10% to chlorophyl a/b binding proteins and 7% to photosystem I and II sequences. Among the remaining 132 ESTs, 76 were tentatively identified by similarity to previously cloned genes in the Blast searches. At least 60 different genes were identified in total. Wheat homologs were present already in GenBank for only nine of these genes. Two sequences were similar to previous wheat entries, but had higher Blast hits with accessions from other species. No corresponding wheat entries were present in GenBank for the remaining 49 genes. Enzymes involved in glycolysis, gluconeogenesis, carbohydrate and fatty acid metabolism, nucleotide and protein biosynthesis were identified. ESTs for structural proteins included histone H3 and beta-tubulin. In addition, a Cf2-like EST was identified that showed high derived amino acid similarity to both an Arabidopsis and a barley sequence. Cf2 conditions resistance to the fungus Cladosporium fulvum in tomato but its function in wheat remains unknown.