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ARS Home » Research » Publications at this Location » Publication #106589

Title: BLOOD SAMPLING AND SURGICAL TECHNIQUES.

Author
item Yen, Jong Tseng

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 1/4/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary: No Interpretive Summary required for book chapters.

Technical Abstract: Blood is a transport medium of nutrients, metabolites, hormones, and other substances in the pig. Properly obtained blood samples offer a means for accurately monitoring the response of blood constituents to dietary factors and the nutritional status of a pig. Blood samples can be collected from a pig by acute venipuncture or chronic catheterization. Surgically prepared pigs provide a powerful tool for investigating digestion and absorption of nutrients in swine. They are particularly useful for studies relating to the kinetics of nutrient digestion and absorption. The use of surgically prepared pigs indicates an intensive type of research and limits the number of animals that can be employed for the experiment. Because of their capacity for control observations and repeat measurements of the phenomenon under investigation, less surgically prepared animals are needed as compared with intact animals. This chapter provides a brief description of fblood sampling and surgical techniques for nutritional studies in the pig. The information should be used only as a guide. The surgical techniques may have to be modified in most cases to accommodate the objectives of certain research, the availability of surgical equipment and supplies, the variation in the size of animals, and the unavoidable anatomical deviation among pigs even of the same breed, sex, and size. Nevertheless, the surgically prepared pig is an extremely valuable tool for nutritional research, and, in some cases, the use of such pig is the only way to obtain certain types of nutritional data.