|Cox, Nelson - Nac|
Submitted to: American Society of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: 3/1/1999
Publication Date: N/A
Citation: Reeves, D.E., Cray, P.J., Bailey, J.S., Stern, N.J., Cox Jr, N.A., Ladely, S.R., Pullen, W. 1999. Mucosal competitive exclusion to reduce salmonella in swine. American Society of Animal Science. 77 (Suppl 1) 46 128-129
Technical Abstract: In two separate trials, a mucosal competitive exclusion culture (MCES) was studied for its effectiveness in reducing Salmonella in suckling pigs. The MCES culture was derived from the cecum of a 6 week-old pig known to be free of Salmonella. MCES was produced in large scale, adjusted to approximately 10(9) CFU/ml, and frozen at -70 degrees until use. In trial one (T1), pigs were given 5ml MCES by oral gavage within 6 hours post- farrowing (PF) and again at 24 hours PF. Control pigs were given 5 ml sterile PBS by oral gavage at same time interval. At 48 hours PF, pigs were inoculated intranasally with 1 ml of S. choleraesuis (10(3) CFU/ml). In trial two (T2), all treatments were the same as T1, except that the challenge dose of S. choleraesuis was increased to 10(6) CFU/ml. Fecal samples were obtained from sows at weekly intervals prior to farrowing and from suckling pigs and sows at daily intervals after farrowing for culture. At day 7 PF necropsy samples of tonsil, lung and lymph nodes, liver, spleen, colon, cecum and ileocecal junction were obtained aseptically from each pig and qualitatively cultured. A cecal fecal sample was quantitatively cultured. No clinical signs of salmonellosis were apparent in either trial nor was S. choleraesuis recovered from fecal samples in either trial. In T1, 9.5% of the tissues were positive from the MCES treated pigs versus 21% positive tissues from the control pigs.