|Jarret, Robert - Bob|
Submitted to: Biotechnology in Agriculture and Forestry
Publication Type: Book / chapter
Publication Acceptance Date: 11/1/1999
Publication Date: 2/1/2000
Citation: Li Z., Jarret, R.L., Cheng, M., Xing, A., Demski, J.W. Biotechnology in agriculture and forestry; Title of Chapter: Transgenic peanut (arachis hypogae). Biotechnology in agriculture and forestry, 2000. pp 208-224 Vol. 46. Transgenic crops sprinter-verlag berlin (2000) Interpretive Summary: This is a Book/Chapter, no interpretive summary required.
Technical Abstract: This book chapter briefly reviews previous research with peanut in the area of genetic transformation, including Agrobacterium-mediated gene transfer techniques. Detailed and previously unreported protocols are presented for the regeneration of cultivated peanut from isolated mesophyll cell protoplasts and for the transformation of these protoplasts with plasmid vectors containing marker genes or gene expression cassettes containing various virus coat protein genes. The chapter also contains the results of a series of experiments that resulted in the successful transformation of peanut with a strain of Agrobacterium tumefaciens harboring a vector containing a gene expression cassette that included the nucelocapsid protein from an isolate of the tomato spotted wilt virus.