Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 2/18/2002
Publication Date: 1/20/2003
Citation: Johnston, D. Methodologies for assaying the hydrolysis of cellulose by cellulases. Book Chapter in Handbook of Food Enzymology. Eds. Whitaker/voragen/wong. Chapter 61:p.761-770. 2003. Interpretive Summary: NO INTERPRETIVE SUMMARY IS REQUIRED
Technical Abstract: The methods described here are generally regarded as the most useful or commonly employed assays for measuring the hydrolysis of cellulose by cellulases. Many of the assays are useful from an empirical standpoint, and can not be used to compare results between laboratories. An attempt has been made to include methods applicable to a variety of research interests. The methods are divided into six categories: Viscosity, Reducing Group, Chromophore and Fluorescent group release, Chromatographic, Coupled enzymes and Special methods (discussion only).