Submitted to: Microbiology and Culture Collections
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/1/1999
Publication Date: N/A
Interpretive Summary: A new yeast was described some years ago but its characteristics seemed atypical when compared with neighboring species. In order to resolve the apparent contradictions, the yeast was compared with all other known yeasts from a gene sequence database developed at NCAUR. This comparison of genetic material showed the yeast was actually a new species, but the confusion arose because the describing author distributed the wrong culture to several culture collections. This study demonstrated the remarkable taxonomic resolution of microbial species that is possible through comparison of gene sequences.
Technical Abstract: IFO 10103 and IFO 10932 (=JCM 2321) were received as Candida agrestis RIFY 4611T (= No. 611) but formed spheroidal to ovoidal chlamydospores and were morphologically similar to Candida pulcherrima, the anamorph of Metschnikowia pulcherrima. However, these strains differed from M. pulcherrima in lactate assimilation, growth at 34 degrees C, and pulcherrimin pigmentation. An analysis of domain D1/D2 26S rDNA sequences from currently accepted ascomycetous yeasts demonstrated that the strains represent a new species, which is described here as Candida kofuensis sp. nov. Phylogenetic placement of this new species in the Metschnikowia clade was further analyzed from 18S rDNA sequence divergence.