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ARS Home » Midwest Area » Madison, Wisconsin » U.S. Dairy Forage Research Center » Research » Publications at this Location » Publication #104708
Title: ORGANIC ACID PRODUCTION FROM ALFALFA FIBER FRACTIONS BY SIMULTANEOUS SACCHARIFICATION AND FERMENTATION

Author
item SREENATH, HASSAN - UNIV OF WISCONSIN MADISON
item Koegel, Richard
item MOLDERS, ANA - UNIV DE VIGO SPAIN
item STRAUB, RICHARD - UNIV OF WISCONSIN MADISON

Submitted to: Biotechnology for Fuels and Chemicals Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 5/6/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Alfalfa fiber was simultaneously saccharified and fermented (SSF) to lactic acid with and without pretreatment by Lactobacillus plantarum, L. delbrueckii, L. xylosus, and L. pentoacetic. We obtained 46.4 g/L lactic acid and 5.0 g/L acetic acid from L. plantarum , whereas L. delbrueckii produced 35.0 g/L lactic acid and 6.0 g/L acetic acid from 100 g/L alfalfa fiber without pretreatment. Similarly from L. xylosus and L. pentoaceticus 18.0 g/L lactic acid and 12.0 g/L acetic acid, and 16 g/L lactic acid and 15.0 g/L acetic acid were respectively produced from 100 g/L unpretreated alfalfa fiber. With SSF of 100 g/L of pretreated alfalfa residue, L. delbrueckii and L. plantarum produced 59 g/L lactic acid and 6.0 g/L acetic acid and, 57 g/L lactic acid and 6.6 g/L acetic acid respectively. Whereas L. xylosus and L. pentoaceticus produced 39 g/L lactic acid and 7.7 g/L acetic acid and, 30 g/L lactic acid and 5 g/L acetic acid respectively from m100 g/L of SSF pretreated alfalfa residue. With SSF of the soluble hemicellulose alfalfa fraction (containing 20 g/L solids), we obtained 12 g/l lactic acid and 6 g/L acetic acid using L. plantarum. Other Lactobacillus cultures produced 2 to 6 g/L lactic acid and 1.5 to 6.4 g/L acetic acid from soluble alfalfa fraction.