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Title: ANALYSIS OF A BORDETELLA BRONCHISEPTICA DERMONECROTIC TOXIN MUTANT IN SWINE

Author
item Register, Karen
item Brockmeier, Susan
item MAGYAR, TIBOR

Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The dermonecrotic toxin (DNT) of B. bronchiseptica is proposed to be a major virulence factor in swine atrophic rhinitis. However, its role during infection in vivo has not been definitively assessed. We constructed a DNT mutant from a virulent swine isolate by replacement of 1.8 Kb of the dnt gene with a kanamycin resistance cassette. Groups of 6 Caesarean-derived, colostrum-deprived piglets were inoculated at 7 days of age with the parent strain, the DNT mutant, or PBS. At 28 days PI, all pigs were necropsied. Results of nasal washes indicate that the mutant was significantly impaired in its ability to colonize the nasal cavity, both at 7 and 28 days PI (p