Submitted to: World Congress International Society for Fat Research
Publication Type: Abstract only
Publication Acceptance Date: 10/7/1999
Publication Date: N/A
Citation: Interpretive Summary:
Technical Abstract: It is important to find a new use for the abundance of annual vegetable oils in the U.S. My group has been investigating value-added products from vegetable oils. One of the products is hydroxy fatty acid. Microbial enzymes can produce not only mono hydroxy but also di- or tri-hydroxy fatty acids. We discovered that a new microbial isolate, Pseudomonas aeruginosa PR3 produced 7S,10S-dihydroxy-8(E)-octadecenoic acid (DOD) from oleic acid through an intermediate, 10S-hydroxy-8(E)- octadecenoic acid. We also found that this PR3 system converted ricinoleic acid to a new compound, 7,10,12-trihydroxy-8(E)-octadecenoic acid with the same reaction mechanism. Furthermore, we discovered that another new microbial isolate, Clavibacter sp. ALA2 produced 12,13,17- trihydroxy-9(Z)-octadecenoic acid (THOA) from linoleic acid. The structures of these hydroxy unsaturated fatty acids resemble those of plant self-defense substances. On anti-pathogens activity tests, DOD inhibited the growth of C. albican but did not inhibit the growth of A. flavus, A. fumigatus, C. neoformans, and M. infracellulare. We also found that at 200 ppm concentration, THOA inhibited growth of some plant pathogenic fungi such as wheat powdery mildew and wheat leaf rust. It seems that the activity against specific pathogens depends on the position of hydroxy groups on the molecule.