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Title: DEVELOPMENT OF SEMICONGENIC LINE 0 B13B13 AND B21B21 CHICKENS FREE OF ENDOGENOUS VIRUS THAT PRODUCE MHC RESTRICTED CTL RESPONSES TO AVL.

Author
item Hunt, Henry
item Bacon, Larry
item PAYNE, WILLIAM - MICHIGAN STATE UNIVERSITY
item SALTER, DONALD - UNIV OF WEST ALABAMA

Submitted to: Avian Diseases
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/6/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary: This paper describes the development of a new genetic stock (line) of chickens. This line is fixed for specific host proteins (B13 Mhc) that are responsible for specific immune responses and lacks the endogenous viral genetic elements that are present in all commercial lines. This line demonstrated the role of Mhc and endogenous elements in altering the specific immune response to the avian leukosis virus (ALV), an important viral infection of chickens. With this new genetic tool we can now investigate the interaction between the host protiens expressed and response to ALV. This will help the poultry industry to select for production of chickens with an increased immune response to this economically important virus, ALV.

Technical Abstract: Line 0 chickens are purposely not inbred and have been selected for the absence of avian leukosis virus related subgroup E (ALVE) genes and homozygosity for the B21 Mhc haplotype (line 0-B21). In this paper we describe the development of a semicongenic line containing the B13 haplotype from line P, i.e. line 0.P-13. The B13 haplotype was derived from mthe 15I5 B congenic inbred line 15.P-13, a line in which also contains ALVE genes 1, 3, 6, and 15. When line 0.P-13 chickens are challenged with subgroup A (RAV-1) avian leukosis virus (ALV) they produce a significant cytotoxic T lymphocyte (CTL) response that is antigen specific and restricted to the B13 haplotype. In contrast line 15.P-13 chickens produce a weak CTL response after ALV challenge, presumably due to expression of ALVE genes. The chickens from lines 0-B21, 0.P-13 and 15.P-13 are valuable genetic stocks for resolving the effects of ALVE and B haplotype genes on immune response to ALV.