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ARS Home » Plains Area » Miles City, Montana » Livestock and Range Research Laboratory » Research » Publications at this Location » Publication #101539
Title: DETERMINATION OF THE STRINGENCY OF DNA MICROSATELLITE MARKER GENOTYPES FOR USE IN INDIVIDUAL ANIMAL IDENTIFICATION

Author
item STOCKBURGER, E - COLORADO STATE UNIVERSITY
item GREEN, R - COLORADO STATE UNIVERSITY
item WOOD, W - COLORADO STATE UNIVERSITY
item HOLM, T - PERKIN ELMER AGGEN
item Macneil, Michael
item SCHAFER, D - COLORADO STATE UNIVERSITY
item YEMM, R - COLORADO STATE UNIVERSITY
item BERG-RAMSEY, J - COLORADO STATE UNIVERSITY

Submitted to: Beef Research Report
Publication Type: Proceedings
Publication Acceptance Date: 4/1/1999
Publication Date: N/A
Citation: STOCKBURGER, E.M., GREEN, R.D., WOOD, W.O., HOLM, T., MACNEIL, M.D., SCHAFER, D.W., YEMM, R.S., BERG-RAMSEY, J. DETERMINATION OF THE STRINGENCY OF DNA MICROSATELLITE MARKER GENOTYPES FOR USE IN INDIVIDUAL ANIMAL IDENTIFICATION. BEEF RESEARCH REPORT. 1999. p. 209-216.

Interpretive Summary: Progress in gene mapping has provided the beef cattle industry with microsatellite DNA markers useful for genotyping. DNA genotypes could provide the basis for unique individual animal identification (ID). Unique animal ID is needed to provide carcass feedback information to producers for use in genetic improvement programs. This project tested 9 markers suggested by the International Society of Animal Genetics (ISAG) to prove their efficacy in identifying individual animals. To test the markers, Hereford cattle (n=90) were sampled from 3 sources. The cattle population was unique because most of the animals were inbred (Fx from 0 to 52%), which provided a more stringent test of the markers. Whole blood was collected from the animals and DNA was extracted from each sample. Each animal was genotyped with the 9 ISAG markers (BM1824, SPS115, ETH3, ETH10, ETH225, TGLA122, TGLA53, TGLA126, and INRA23). To determine the ability of these 9 markers to provide a unique genotype for each animal, allelic frequencies for each marker were calculated. Assuming Hardy-Weinberg equilibrium, these frequencies were used to determine the frequency of the most common cumulative genotype over all 9 loci (4.3 x 10**-5). Using this frequency, the probability that any two random individuals in this population possess identical genotypes would be no greater than 2.0 x 10**-9. The total number of possible genotypes with the 9 markers was also calculated (3.1 x 10**10). These data indicate that these 9 microsatellite markers are effective for individual animal ID and suggest that the cattle industry could pursue microsatellite DNA genotyping as the basis for an individual animal ID system.

Technical Abstract: Progress in gene mapping has provided the beef cattle industry with microsatellite DNA markers useful for genotyping. DNA genotypes could provide the basis for unique individual animal identification (ID). Unique animal ID is needed to provide carcass feedback information to producers for use in genetic improvement programs. This project tested 9 markers suggested by the International Society of Animal Genetics (ISAG) to prove their efficacy in identifying individual animals. To test the markers, Hereford cattle (n=90) were sampled from 3 sources. The cattle population was unique because most of the animals were inbred (Fx from 0 to 52%), which provided a more stringent test of the markers. Whole blood was collected from the animals and DNA was extracted from each sample. Each animal was genotyped with the 9 ISAG markers (BM1824, SPS115, ETH3, ETH10, ETH225, TGLA122, TGLA53, TGLA126, and INRA23). To determine the ability of these 9 markers to provide a unique genotype for each animal, allelic frequencies for each marker were calculated. Assuming Hardy-Weinberg equilibrium, these frequencies were used to determine the frequency of the most common cumulative genotype over all 9 loci (4.3 x 10**-5). Using this frequency, the probability that any two random individuals in this population possess identical genotypes would be no greater than 2.0 x 10**-9. The total number of possible genotypes with the 9 markers was also calculated (3.1 x 10**10). These data indicate that these 9 microsatellite markers are effective for individual animal ID and suggest that the cattle industry could pursue microsatellite DNA genotyping as the basis for an individual animal ID system.