Submitted to: American Society of Sugar Cane Technologists
Publication Type: Abstract Only
Publication Acceptance Date: 5/10/1999
Publication Date: N/A
Citation: Pan, Y., Legendre, B.L., Burner, D.M. 1999. Marker-assisted sugarcane breeding [abstract]. Sugar y Azycar. 94(6):27.
Technical Abstract: Molecular markers are being developed to assist in the selection of interspecific sugarcane hybrids. A random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) marker OPA-11-366 was identified that was unique to sugarcane. This marker was used in conjunction with visual selection to screen for true hybrids among two progeny population derived from crosses between a S. spontaneum clone Djatiroto and two sugarcane varieties, LCP 85-384 and CP 62-258. Based on two sugarcane-related morphological traits, namely, larger stalk diameter and the presence of spines, 42 putative hybrids were selected. Total nucleic acids were extracted from the leaf tissue and analyzed by RAPD-PCRs using primer OPA-11. Thirty-three of the 42 putative hybrids produced the 366 bp fragment that also hybridized strongly with the OPA-11-366 marker labeled with radioactive P-32, thus representing true F1 hybrids. The remaining 9 putative hybrids represented selfed progeny of the female S. spontaneumclone Djatiroto as they did not amplify the 366 bp fragment. The utilization of molecular markers, as was documented in this study, will allow selection of true hybrids at the seedling stage which should reduce the operation cost as well as save field space.