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Title: LINKAGE ASSIGNMENT OF THE LAST UNMAPPED ERYTHROCYTE ANTIGEN, EAF

Author
item Kappes, Steven - Steve
item HINES, H - OHIO STATE UNIVERSITY
item PENEDO, M - UNIV. CALIFORNIA, DAVIS
item Keele, John

Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/8/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary: Eleven erythrocyte antigens (blood groups) have traditionally been used for parentage testing in cattle and all but one, erythrocyte antigen F, have been assigned to chromosomal regions by linkage information. Determining the chromosomal location of erythrocyte antigens can enhance the transition from erythrocyte antigens to DNA markers for parentage testing. DNA markers located near the erythrocyte antigens can be used to join the old blood group data and new DNA marker data within certain livestock pedigrees provided that both types of markers are used on some of the same animals. Erythrocyte antigen F was assigned to the top portion (centromeric end) of bovine chromosome 17, between the DNA markers BM2220 and INRA193.

Technical Abstract: Description: Eleven erythrocyte antigen systems (EA) have traditionally been used for parentage testing in cattle and all but one, EAF, have been assigned to chromosomal regions by genetic linkage information. EAF has been reported as being linked to EAS and Pi2 although the statistical support for the linkage was not very strong. Statistical support for linkage was slightly higher with a multipoint analysis when comparing the presence or absence of linkage between [Pi2-EAS] and EAF. In contrast, two independent reports provided convincing evidence against EAF and EAS linkage at recombination frequencies (RF) of 0.35 and 0.44 respectively. Genotyping: We have genotyped the EAF blood group system in 19 cow families from the USDA-MARC bovine reference population. The EAF genotypes were determined by using internationally standardized, functionally monospecific haemolytic reagents prepared by absorption of alloimmune cattle sera according to standard procedures. All genotypic information was entered into a relational database. Linkage Analysis and Chromosomal Location: Linkage analysis using CRI- MAP was done as previously reported. The twopoint analysis was performed against approximately 1450 previously linked markers. Twopoint linkages were detected between fifteen chromosome 17 markers and EAF (119 informative meioses) with a range in lodscores from 3.40 to 15.95. The marker with closest linkage to EAF was BMS2220. Evaluation of 42 co- informative meioses excludes linkage between EAS and EAF at theta